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Human IL-17 RC Protein, Fc Tag (MALS verified)

Human IL-17 RC Protein, Fc Tag (MALS verified)
别名: IL-17 RC,IL-17RC,IL17Rhom,IL-17 receptor C,IL-17RL,ZcytoR14
储存条件: -20℃ 
Human IL-17 RC Protein, Fc Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILC-H5259-5 100ug 现货 0.00
ZY-ILC-H5259-100ug 100ug 现货 3000.00 3000
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熔点:
密度:
储存条件: -20℃

 

Human IL-17 RC Protein, Fc Tag (MALS verified)

分子别名(Synonym)

IL-17 RC,IL-17RC,IL17Rhom,IL-17 receptor C,IL-17RL,ZcytoR14

表达区间及表达系统(Source)

Human IL-17 RC, Fc Tag  is expressed from human 293 cells . It contains AA Leu 21 - Ala 465.

Predicted N-terminus: Leu 21

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 75.9 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) SDS-PAGE gel

Human IL-17 RC, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human IL-17 RC, Fc Tag is more than 90% and the molecular weight of this protein is around 200-230 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL17A, Tag Freeat 2 μg/mL (100 μL/well) can bind Human IL-17 RC, Fc Tag with a linear range of 5-160 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human IL-17F, His Tag  at 2 μg/mL (100 μL/well) can bind Human IL-17 RC, Fc Tag with a linear range of 0.02-0.25 μg/mL (Routinely tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human IL-17A Protein, Tag Free  immobilized on CM5 Chip can Human IL-17 RC, Fc Tag with an affinity constant of 72.8 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

Biotinylated Human SPR

Biotinylated Human IL-17A Protein, His,Avitag™  captured on Biotin CAP - Series S sensor Chip can bind Human IL-17 RC, Fc Tagwith an affinity constant of 40.9 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human IL17A, His,Avitag on SA Biosensor, can bind uman IL-17 RC, Fc Tag with an affinity constant of 13.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag on NTA Biosensor, can bind Human IL-17 RC, Fc Tag  with an affinity constant of 92.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human IL-17F, His Tag on NTA Biosensor, can bind Human IL-17 RC, Fc Tag with an affinity constant of 100 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 

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