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Human CD27 Ligand / CD70 Protein, Mouse IgG2a Fc Tag, low endotoxin

Human CD27 Ligand / CD70 Protein, Mouse IgG2a Fc Tag, low endotoxin
别名: CD70,CD27LG,TNFSF7,TNFSF7G,CD27L
储存条件: -20℃ 
Human CD27 Ligand / CD70 Protein, Mouse IgG2a Fc Tag, low endotoxin
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-CDL-H525a-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Human CD27 Ligand / CD70 Protein, Mouse IgG2a Fc Tag, low endotoxin

分子别名(Synonym)

CD70,CD27LG,TNFSF7,TNFSF7G,CD27L

表达区间及表达系统(Source)

Human CD27 Ligand, Mouse IgG2a Fc Tag, low endotoxinis expressed from human 293 cells. It contains AA Gln 39 - Pro 193 

Predicted N-terminus: Glu

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a mouse IgG2a Fc tag at the N-terminus.

The protein has a calculated MW of 44.0 kDa. The protein migrates as 50-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>85% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human CD27 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525a) SDS-PAGE gel

Human CD27 Ligand, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD27 at 2 μg/mL (100 μL/well) can bind Human CD27 Ligand, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.2-6 ng/mL (QC tested).

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