Human 4-1BB Ligand / TNFSF9 (71-254) Protein, Fc Tag, active trimer (MALS verified)

Human 4-1BB Ligand / TNFSF9 (71-254) Protein, Fc Tag, active trimer (MALS verified)

分子别名（Synonym）

4-1BB Ligand,TNFSF9,CD137L

表达区间及表达系统（Source）

Human 4-1BB Ligand (71-254), Fc Tag, active trimer is expressed from human 293 cells . It contains AA Arg 71 - Glu 254 trimer Design.

Predicted N-terminus: Pro

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 87.5 kDa. The protein migrates as 150-200 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Human 4-1BB Ligand (71-254), Fc Tag, active trimer on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

The purity of Human 4-1BB Ligand (71-254), Fc Tag, active trimer is more than 90% in HP-SEC, and the molecular weight of this protein is around 185-225 kDa verified by SEC-MALS.

Report

 

活性（Bioactivity）-ELISA

Immobilized Human 4‑1BB, His Tag at 2 μg/mL (100 μL/well) can bind Human 4-1BB Ligand (71-254), Fc Tag, active trimer with a linear range of 0.2-10 ng/mL (QC tested).

Protocol

活性（Bioactivity）-BLI

Loaded Human 4-1BB Ligand (71-254), Fc Tag, active trimeron Protein A Biosensor, can bind Cynomolgus / Rhesus macaque 4-1BB, His Tag with an affinity constant of 0.103 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性（Bioactivity）-Cell based assay

Human 4-1BB Ligand (71-254), Fc Tag, active trimer induce IL-8 secretion in HT1080 human CD137 cell line. The EC50 for this effect is 0.079 μg/mL (Routinely tested).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！